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| Registros recuperados: 24 | |
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| B,Jayashree; Ferguson,Morag; Ilut,Dan; Doyle,Jeff; Crouch,Jonathan H. |
| Peanut is an important legume crop across the world. However, in contrast to most legume crops, groundnut lacks taxonomic proximity to any major model genome. A relatively large number of genomic sequences were generated from groundnut as part of a microsatellite marker development project. In the current study, a total of 1312 sequences were analyzed of which 448 contained microsatellite motifs. All sequences (GenBank Accessions: BZ999351-CC000573) were analyzed after clustering for possible similarity with publicly available sequences from Arabidopsis, Lotus, soybean and Medicago. At least 39% of the sequences analyzed had significant BLAST similarities with sequences from the four databases searched, of which nearly half (47%) found significant... |
| Tipo: Journal article |
Palavras-chave: Arachis hypogaea; Codon usage; Gene-based markers; Peanut; SSR markers. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300002 |
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| Bombonato,Juliana Rodrigues; Bonatelli,Isabel Aparecida Silva; Silva,Gislaine Angélica Rodrigues; Moraes,Evandro Marsola; Zappi,Daniela Cristina; Taylor,Nigel P.; Franco,Fernando Faria. |
| Abstract The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cactaceae; Cereus; Cross-genera; SSR markers; Transferability. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100087 |
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| Bergamaschi,Antonio C.B.; Lama,Marco A. Del. |
| Abstract Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to northern Argentina. Eight species-specific microsatellite loci were used for genotyping 96 adult wasps (one female per nest) sampled at five sites in Brazil. The analysis of allelic richness and private alleles indicated high genetic diversity in the populations sampled. Pairwise comparisons using the Fst and Dest indices revealed significant differentiation for all, but one pair of populations. Fst, Dest, AMOVA and assignment test... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Population genetics; Wasps; Crabronidae; SSR markers. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400519 |
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| Guidugli,Marcela Corbo; Accoroni,Klaus Alvaro Guerrieri; Mestriner,Moacyr Antonio; Contel,Eucleia Primo Betioli; Martinez,Carlos Alberto; Alzate-Marin,Ana Lilia. |
| Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Jequitibá rosa; Population genetics; SSR markers; Transferability. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100022 |
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| Siqueira,Marcos V. B. M.; Queiroz-Silva,Jurema R.; Bressan,Eduardo A.; Borges,Aline; Pereira,Kayo J. C.; Pinto,José G.; Veasey,Elizabeth Ann. |
| Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H S = 0.52). Cluster analysis and principal component based scatter plotting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Microsatellites; SSR markers; Traditional farming. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100015 |
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| Solano,Jaime; Mathias,Monica; Esnault,Florence; Brabant,Philippe. |
| Background: The object of this work was to determine the genetic and allelic diversity of Solanum species present in Chile, assessing allelic distribution among native varieties and commercial cultivars of Solanum tuberosum ssp. tuberosum L., using microsatellite markers. Results: A high level of allelic richness was found in the potatoes studied. The seven microsatellite markers used presented a total of 64 allelic variants among native varieties and commercial cultivars of Solanum tuberosum ssp. tuberosum. The SSR loci generated an average of 9.16 alleles/locus. The group with the highest PIC was that of native varieties collected in the Chiloe archipelago. The high PIC values found indicate that the native varieties from Chiloe have a low level of... |
| Tipo: Journal article |
Palavras-chave: Dendrogram; Germplasm; SSR markers. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600008 |
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| Kanawapee,Nantawan; Sanitchon,Jirawat; Srihaban,Pranee; Theerakulpisut,Piyada. |
| Thirty rice cultivars were evaluated for salinity tolerance during the seedling stage and were divided into five tolerance groups including tolerant (T), moderately tolerant (MT), moderately susceptible (MS), susceptible (S) and highly susceptible (HS) which comprised 5, 10, 9, 4 and 2 cultivars respectively. Genetic diversity of all rice cultivars was evaluated using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers. The cultivars were evaluated for polymorphisms after amplification with 20 random decamer primers and 20 SSR primer pairs. A total of 161 RAPD markers and 190 SSR alleles were produced which revealed 68.94% and 89.47% polymorphism respectively. Mean genetic similarity coefficient was 0.82 for RAPD and 0.70 for... |
| Tipo: Journal article |
Palavras-chave: Genetic similarity; RAPD markers; Rice; Salinity tolerance; SSR markers. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600002 |
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| Yoon,Min-Young; Moe,Kyaw Thu; Kim,Dae-Young; Rho,Il-Rae; Kim,Su; Kim,Ki-Taek; Won,Mi-Kyoung; Chung,Jong-Wook; Park,Yong-Jin. |
| In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a... |
| Tipo: Journal article |
Palavras-chave: Genetic diversity; Population structure; SSR markers; Strawberry. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200006 |
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| Cuyeu,Romina; Rosso,Beatriz; Pagano,Elba; Soto,Gabriela; Fox,Romina; Ayub,Nicolás Daniel. |
| Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SSR markers; Genetic diversity; Flow cytometry; Tall fescue; Genetic resources. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200014 |
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| Henkrar,Fatima; El-Haddoury,Jamal; Ouabbou,Hassan; Nsarellah,Nasserlehaq; Iraqi,Driss; Bendaou,Najib; Udupa,Sripada Mahabala. |
| ABSTRACT It has been argued that genetic diversity in crop varieties has been on the decline in recent times due to plant breeding. This can have serious consequences for both the genetic vulnerability of crops and their plasticity when responding to changes in production environments. It is, therefore, vital for plant breeding programs to maintain sufficient diversity in the cultivars deployed for multi-period cultivation. In this study, to understand the temporal genetic diversity in durum wheat, 21 improved durum wheat cultivars released in Morocco, since 1956 and five exotic cultivars currently used in crossing programs were analyzed using 13 microsatellite markers. The analysis revealed a total of 44 alleles and average genetic diversity of 0.485 with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum turgidum L. subsp. durum; Cultivar characterization; SSR markers. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000200134 |
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| Torres,Adalgisa Ribeiro; Grunvald,Anna Karolina; Martins,Talita Busulini; Santos,Maria Aparecida dos; Lemos,Noélle Giacomini; Silva,Luis Antônio Stabile; Hungria,Mariangela. |
| Biological nitrogen fixation (BNF) has global economic and environmental importance, but has often not been considered in soybean [Glycine max (L.) Merrill] breeding programs. Knowing the genetic diversity and structure of a population within a germoplasm represent a key step for breeding programs. This study aimed at determining the structure of the population and diversity of soybean with regard to BNF and protein content in grain. In total, 191 accessions were evaluated, including 171 commercial soybean cultivars, developed and released by public institutions and private companies in Brazil, and 20 ancestral lines. The genotypes were chosen to represent four genetic groups: 128 Brazilian public genotypes, 20 exotic, and 43 genotypes from private... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BNF; Bradyrhizobium; Glycine max; SSR markers; Symbiosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100047 |
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| Gomez Selvaraj,Michael; Narayana,Manivannan; Schubert,A. Michael; Ayers,Jamie L; Baring,Michael R; Burow,Mark D. |
| Bulked segregant analysis was used to identify simple sequence repeat (SSR) markers associated with pod and kernel traits in cultivated peanut, to permit rapid selection of superior quality genotypes in the breeding program. SSR markers linked to pod and kernel traits were identified in two DNA pools (high and low), which were established using selected F2:6 recombinant individuals resulting from a cultivated cross between a runner (Tamrun OL01) and a Spanish (BSS 56) peanut. To identify quantitative trait loci (QTLs) for pod and kernel-related traits, parents were screened initially with 112 SSR primer pairs. The survey revealed 8.9% polymorphism between parents. Of ten SSR primer pairs distinguishing the parents, five (PM375, PM36, PM45, pPGPseq8D9, and... |
| Tipo: Journal article |
Palavras-chave: Maturity; Oil quantity; Quality; SSR markers. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200003 |
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| Registros recuperados: 24 | |
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